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Efficient and Soluble Expression of α Protein of Clostridium perfringens and Preparation


Animal Husbandry and Feed Science, 2017, 9 ( 5 ) : 284 -2 8 8 , 305 DOI:10.19578/j. cnki. ahfs.2017.05.007 Bfici^nt and Soluble Expression of a Protein of Closfr/d/i/m perfr/ngens and Preparation of GeneticEng^ neering Subunit Vaccine Sun Yu , Yang Lin , W ang Chuanbin , Dong H ao , Q u Ping , Zhao Bolin , H u Dongmei , Yang Tianyi , Song Xiaohui China Animal Disease Control Center, Beijing 102600, China Abstract ge?g. Key words COstridium pejfringens ;a [ Objective] The paper was to develop genetic engneering vaccine that can express a exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clostridium pejfringens. [ M ethod] Efficiently expressed soluble rec from Escherichia coli expression system by optimizing codon , removing signal peptide , selecting sequences with better hydrophilicity and antigenicity , and optimizing expression conditions. [ Result] Mice obtained higher serum antibody level when immunized by a protein , and the immune protection rates a ainst type A , type B , type C and type D C. pejfringegs were 100% , 90% , 85% and 90% , respectively. The antibody titer of mice within 7 -14 d after the third immunization reached the peak. [ Conclusion ] The a protein has good immunogenicity , and can be further used to develop genetic engineering subunit vaccines for preventing C. perfrinprotein ;Soluble expression and purification ;Genetic engineering subunit vaccine vent and control th

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